Mouse anti Human CD10 FITC - CD19 PE
ELISA, WB
Reactivity: Human
ELISA, WB
PE,FITC
Host: Chicken
Polyclonal
Catalog No. ABIN2763665
Quick Overview for Mouse anti Human CD10 FITC - CD19 PE (ABIN2763665)
Target
MME
(Membrane Metallo-Endopeptidase (MME))
Reactivity
Human
Host
Chicken
Clonality
Polyclonal
Conjugate
PE,FITC
Application
ELISA, Western Blotting (WB)
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Specificity
- The GFP antiserum is directed against native green fluorescent protein from A.victoria jellyfish. The antibody recognises not only the native GFP, but also recombinant protein (E.Coli), native GFP-fusion proteins and all variants of GFP tested. Cross reactivity with E.coli. proteins is minimal.
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Cross-Reactivity (Details)
- Aequoria Victoria (native) GFP, recombinant (E.Coli) and native GFP-fusion proteins, and all variants of GFP tested.
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Characteristics
- CD10 FITC-CD19 PE,Green fluorescent protein (GFP) is a 27kD protein which was originally identified in the photo organs of Aequorea victoria (A. victoria) jellyfish. GFP is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. In A.victoria, calcium ions bind and activate the protein aequorin causing the release of blue fluorescence, which is then absorbed by GFP resulting in the release of green fluorescence. In the laboratory, the GFP protein has been used extensively as a reporter molecule to label, and study, cellular and subcellular proteins in living cells using a wide range of biological applications, including oncology, cardiovascular diseases, brain research and embryology, just to name a few. For the quantitation of the expression of a specific protein, tagged with GFP in these model systems, antibodies to GFP have proven to be of value in immunoblotting studies and ELISA protocols.
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Purification
- Ammonium sulfate precipitation
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Isotype
- IgG1
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MME (Human) IP-WB Antibody Pair
IP Reactivity: Human IP unconjugated Host: Rabbit, Mouse
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Application Notes
- Western Blotting|ELISA
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Restrictions
- For Research Use only
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Concentration
- 1 mg/mL
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Buffer
- Each vial contains 100 μL 1 mg/mL polyclonal antiserum in 10 mM TRIS buffer pH 8.0, containing 0,15 mM NaCl and 0.02 % sodium azide. The antibodies were purified by ammonium sulfate precipitation.,Each vial contains 100 μL 1 mg/mL polyclonal antiserum in 10 mM TRIS buffer pH 8.0, containing 0,15 mM NaCl and 0.02 % sodium azide.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- RT,4 °C,-20 °C
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Storage Comment
- The antibody may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
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- MME (Membrane Metallo-Endopeptidase (MME))
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Alternative Name
- CD10
Target
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